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Yeast killers

In report one these compounds together killed 100% of yeast in 120 minutes: glycerol monolaurate (GML), propionic acid, Tween 80, sodium benzoate.

1. Pub Med "The anti-yeast activities of a food-grade dilution-stable microemulsion against Candida albicans and Saccharomyces cerevisiae have been studied. The weight ratio of the formulated microemulsion is glycerol monolaurate (GML)/propionic acid/Tween 80/sodium benzoate (SB)/water = 3:9:14:14:24. Results of anti-yeast activity on solid medium by agar diffusion method showed that the anti-yeast activity of the microemulsion at 4.8 mg/ml was comparable to that of natamycin at 0.1 mg/ml as positive control. Results of anti-yeast activity in liquid medium by broth dilution method showed that the growth of both C. albicans and S. cerevisiae was completely inhibited when the liquid medium containing 10(6) cfu/ml was treated with 1.2 mg/ml microemulsion, which was determined as minimum fungicidal concentration. The kinetics of killing results showed that the microemulsion killed over 90% yeast cells rapidly within 15 min and caused a complete loss of viability in 120 min. Among the components, SB and GML had a similar anti-yeast activity, followed by propionic acid, while Tween 80 exhibited no activity and could not enhance the anti-yeast activities of these components, and it was revealed that the anti-yeast activity of the microemulsion was attributed to a combination of propionic acid, GML, and SB. The anti-yeast activity of the microemulsion was in good agreement with the leakage of 260-nm absorbing materials and the observation of transmission electron microscopy, indicating that the microemulsion induced the disruption and dysfunction of the cell membrane." http://www.ncbi.nlm.nih.gov/pubmed/20437043

Research about the four compounds in report 1.

A.

Glycerol monolaurate (GML)"

B.

In human health propionic acid is a digestive acid. I have not yet identified how much is a safe dose, however too much may upset the ph in the digestive flora and tax the gall bladder.

1. Propionic acid"Propanoic acid (from 'propane', and also known as propionic acid) is a naturally occurring carboxylic acid with chemical formula CH3CH2COOH. It is a clear liquid with a pungent odor....Propanoic acid inhibits the growth of mold and some bacteria at the levels between 0.1 and 1% by weight. As a result, most propanoic acid produced is consumed as a preservative for both animal feed and food for human consumption. For animal feed, it is used either directly or as its ammonium salt. The antibiotic Monensin is added to cattle feed to favor propionibacteria over acetic acid producers in the rumen; this produces less carbon dioxide and feed conversion is better. This application accounts for about half of the world production of propanoic acid. Another major application is as a preservative in baked goods, which use the sodium and calcium salts.[2] As a food additive it is approved for use in the EU,[3] USA[4] and Australia and New Zealand;[5] it is listed by its INS number (280) or E number E280." http://en.wikipedia.org/wiki/Propanoic_acid

2. propionic acid "found in chyme (The thick semifluid mass of partly digested food that is passed from the stomach to the duodenum.) and sweat, and one of the products of bacterial fermentation of wood pulp waste; also an important gluconeogenic (a metabolic pathway that results in the generation of glucose) volatile fatty acid synthesized by the ruminal (stomach compartment of cows that digests vegatation) microflora. Its salts (calcium and sodium propionate) are used as mold inhibitors in stock feeds and pharmaceuticals, and in topical antifungal preparations." http://medical-dictionary.thefreedictionary.com/propionic+acid

C. Tween 80 "
D. Dodium benzoate"